FastQCFastQC Report
Tue 9 Sep 2025
Anammox-biofilm-on-GAC_merged.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnammox-biofilm-on-GAC_merged.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106348
Total Bases26.9 Mbp
Sequences flagged as poor quality0
Sequence length233-299
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCACGTA2641024.833565276262835No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA80967.6127430699213905No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA68136.40632640012036No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA40983.85338699364351No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA27192.5567006431714745No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA14781.3897769586640087No Hit
CACGTAGGAGGCGAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA14721.3841351036220708No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA14601.3728513935381954No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA11861.1152066799563698No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA11541.085116786399368No Hit
TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA11101.0437431827584909No Hit
TACGGGGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA10250.9638169029977056No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA9510.8942340241471395No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA9240.8688456764584195No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGTGCGTA8850.8321736186858238No Hit
TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA8570.8058449618234477No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA8350.785158160003009No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA7740.7277993004099749No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA7610.7155752811524428No Hit
TACGTAGGTGTCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCCCGCA7220.6789032233798473No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA7000.6582164215594087No Hit
TACGAACCGTACGAACGTTATTCGGAATCACTGGGCTTAAAGCGTGCGTA6940.6525745665174709No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA6800.6394102380862828No Hit
CACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA6480.6093203445292812No Hit
CACGTAGGAGGCAAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA6400.6017978711400309No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA6220.5848723060142175No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA6000.564185504193779No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA5890.5538421032835597No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA5760.5416180840260277No Hit
TACGAAGGATGCAAGCGTTATCCGGAATTATTGGGTTTAAAGGGTGCGTA5700.53597622898409No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA5560.5228119005529018No Hit
AACGTAGGAGACAAGCGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGTA5110.4804979877383684No Hit
TACGGGCGGGGCGAGCGTTGTTCGGATTCATTGGGTGTAAAGGGTGCGAA5100.47955767856471204No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA5080.47767706021739953No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5000.4701545868281491No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4850.4560499492233046No Hit
TACGAAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA4660.438184074923835No Hit
TACGTAGGGGCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGCGCGCGCA4260.40057170797758307No Hit
TACGTAGGCTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGCA3980.3742430511152067No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA3850.3620190318576748No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA3460.3253469740850792No Hit
CACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGCA3370.31688419152217245No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3030.28491367961785835No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTCACTGGGTGTAAAGGGCGCGCA3020.28397337044420207No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA3020.28397337044420207No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA2950.27739120622860797No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA2910.2736299695339828No Hit
AACGTAGGTGACAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA2780.2614059502764509No Hit
TACGTAGGCAGCAAACGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA2640.24824162184526274No Hit
TACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2570.24165945762966864No Hit
TACGTAGGGGGCAAACGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA2560.24071914845601233No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2480.23319667506676195No Hit
TACAGAGGTGCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA2460.23131605671944935No Hit
GACGTAGGGGGCGAGCGTTGTCCGAAGTGACTGGGCGTAAAGCGCACGTA2380.223793583330199No Hit
TACGTAAGGAGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCTCGTA2350.22097265580923006No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGAGCGTGTA2230.20968894572535451No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA2220.2087486365516982No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA2210.2078083273780419No Hit
TACGGAAGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2190.2059277090307293No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA2180.20498739985707304No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2070.19464399894685372No Hit
TACGTAGGGGACGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGCA2010.18900214390491593No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1950.18336028886297814No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA1940.18241997968932183No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGAGTA1890.17771843382104036No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA1880.17677812464738407No Hit
TACGGGGGGTGCGAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGCGCA1860.17489750630007148No Hit
TACGTAGGACCCGAGCGTTGTCCGGAATTACTGGGTATAAAGGGTGCGCA1820.17113626960544626No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGCGCGTA1800.1692556512581337No Hit
TACGAACTGTGCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA1780.1673750329108211No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA1770.16643472373716478No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1770.16643472373716478No Hit
GACGTAGGGGCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGGGCGCGCA1760.16549441456350847No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA1600.15044946778500773No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA1580.1485688494376951No Hit
CACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA1550.14574792191672623No Hit
TACGTACCGTCCAAACGTTATTCGGAATTACTGGGCTTAAAGGGTACGTA1540.1448076127430699No Hit
TACGTAGGGGGCGAGCGTTGTCCGAAGTTACTGGGCGTAAAGCGCGCGTA1530.1438673035694136No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA1510.14198668522210103No Hit
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAGGAGCACGTA1500.14104637604844475No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1440.13540452100650693No Hit
CACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA1430.13446421183285062No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA1400.13164328431188174No Hit
AACGTAGGAGGCAAGCGTTATCCGGAGTTACTGGGCGTAAAGCGCGCGCA1390.13070297513822546No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGTGCGTA1380.12976266596456915No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA1320.12412081092263137No Hit
TACGGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCACGTA1290.12129988340166248No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGTGCGTA1280.12035957422800617No Hit
TACGGGTGAGGCAAGCGTTGTTCGGAATCACTGGGCTTAAAGCGCGTGTA1270.11941926505434988No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA1270.11941926505434988No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA1260.11847895588069357No Hit
TACGTAGGGGCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCGCGCA1250.11753864670703727No Hit
TACAGAGGCGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCACGTA1150.10813555497047429No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGGGCGTGTA1150.10813555497047429No Hit
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA1150.10813555497047429No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA1100.1034340091021928No Hit
TACAGAGGCGGCAAGCGTTGCTCGGAATTATTGGGCGTAAAGAGCACGTA1100.1034340091021928No Hit
TACGAACCGTCCAAACGTTATTCGGAATTACTGGGCTTAAAGGGTACGTA1080.10155339075488021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)