FastQCFastQC Report
Tue 9 Sep 2025
Liquid-fraction-after-anammox-biofilm-formation_merged.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLiquid-fraction-after-anammox-biofilm-formation_merged.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117571
Total Bases29.7 Mbp
Sequences flagged as poor quality0
Sequence length244-299
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1920616.33566100483963No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1631513.876721300320657No Hit
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCACGTA86957.3955312109278655No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA40843.4736457119527775No Hit
TACGAACCGTACGAACGTTATTCGGAATCACTGGGCTTAAAGCGTGCGTA25692.1850626429986986No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA21651.841440491277611No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA21581.835486642114127No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20761.7657415519133122No Hit
TACGTAGGCTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGCA20221.7198118583664337No Hit
GACGTAGGGGGCGAGCGTTGTCCGAAGTGACTGGGCGTAAAGCGCACGTA18991.6151942230652119No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA17051.4501875462486498No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA16161.3744886068843507No Hit
TACAGAGGTGCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA15381.3081457162055268No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA14381.2230907281557526No Hit
AACGTAGGTGACAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA14171.2052291806653002No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA13491.1473917887914538No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA12231.0402225038487383No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA11730.9976950098238511No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA10860.9236971702205476No Hit
TACGTAGGACCCGAGCGTTGTCCGGAATTACTGGGTATAAAGGGTGCGCA10670.9075367224910905No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA9720.8267344838438051No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGTGCGTA9140.777402590774936No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA8870.754437744001497No Hit
TACGGGCGGGGCGAGCGTTGTTCGGATTCATTGGGTGTAAAGGGTGCGAA8500.7229673984230806No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA8490.7221168485425828No Hit
TACGGGTGAGGCAAGCGTTGTTCGGAATCACTGGGCTTAAAGCGCGTGTA7770.6608772571467454No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGTGCGTA7570.6438662595367906No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA6050.5145826777011339No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA5570.4737562834372422No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA5200.44228593785882575No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA5160.4388837383368348No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA4960.42187274072687997No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA4840.411666142160907No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA4210.35808149968954933No Hit
TACGTAGGGGCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGCGCGCGCA4050.34447270160158544No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3980.33851885243810126No Hit
TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA3790.32235840470864413No Hit
TACGTAGGTGGCGAACGTTGTCCGGATTCACTGGGCGTAAAGCGCGCGCA3470.2951408085327164No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA3420.2908880591302277No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA3190.2713254118787796No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTA2790.23730341665886995No Hit
TACGTAGGGGGCAAACGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA2740.23305066725638124No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA2730.23220011737588353No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2730.23220011737588353No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2680.22794736797339482No Hit
GACGTAGGATGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGAGTTCGTA2560.21774076940742187No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA2520.21433856988543093No Hit
GACGTAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTA2430.20668362096095128No Hit
CACGTAGGAGGCGAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2430.20668362096095128No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA2380.2024308715584625No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA2360.20072977179746704No Hit
TACGAACTGTGCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA2140.18201767442651676No Hit
TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA2120.18031657466552126No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA2080.1769143751435303No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA2080.1769143751435303No Hit
TACGGGGGGTGCGAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGCGCA2060.1752132753825348No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA2000.17010997609954837No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1950.16585722669705966No Hit
TACAGAGACTGCGAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA1930.16415612693606416No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA1910.16245502717506868No Hit
TACGAAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCATGCA1880.15990337753357545No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA1860.15820227777257997No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1850.15735172789208224No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1830.15565062813108674No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA1730.14714512932610932No Hit
TACGTAGGCAGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGAGTGCGTA1730.14714512932610932No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA1710.14544402956511385No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA1680.1428923799236206No Hit
TACGTAGGTGTCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCCCGCA1680.1428923799236206No Hit
TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1670.14204183004312287No Hit
TACGGAGGGTGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA1650.1403407302821274No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGAGCGTGTA1650.1403407302821274No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1640.13949018040162964No Hit
TACGAAGGATGCAAGCGTTATCCGGAATTATTGGGTTTAAAGGGTGCGTA1530.1301341317161545No Hit
TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1460.1241802825526703No Hit
TACGTAGGTCACGAACGTTATTCGGAATCACTGGGCTTAAAGGGCGCGTA1450.12332973267217257No Hit
TACGTAGGGGACGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGCA1420.12077808303067933No Hit
TACGAAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1420.12077808303067933No Hit
TACGTAGGTGGCTAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA1350.11482423386719515No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA1320.11227258422570191No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCCCGCA1250.10631873506221773No Hit
GACGTAGGGGGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGTGTA1240.10546818518171999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)