FastQCFastQC Report
Tue 9 Sep 2025
Anammox-seeding-sludge_merged.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAnammox-seeding-sludge_merged.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108871
Total Bases27.5 Mbp
Sequences flagged as poor quality0
Sequence length249-299
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCACGTA2538923.32025975696007No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA86927.983760597404268No Hit
TACGGGGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA31882.9282361694115053No Hit
TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA24532.253125258333257No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA22182.03727347043749No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA18811.7277328214124972No Hit
TACGAAGGATGCAAGCGTTATCCGGAATTATTGGGTTTAAAGGGTGCGTA16821.5449476903858694No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA16651.5293328801976651No Hit
TACGTAGGCTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTACGCA16371.5036143692994461No Hit
TACGTAGGTGTCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCCCGCA15971.4668736394448476No Hit
CACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA15061.383288479025636No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA14651.3456292309246725No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA12471.1453922532171101No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA12321.1316144795216356No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA11111.020473771711475No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA9880.9074960274085844No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGAGTA8690.7981923560911538No Hit
CACGTAGGAGGCGAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA8320.7642071809756501No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA8140.7476738525410807No Hit
TACGAAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA7920.7274664511210515No Hit
CACGTAGGAGGCAAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA7550.6934812760055479No Hit
TACGTAGGGGGCAAACGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA7450.6842960935418982No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6980.6411257359627449No Hit
TACGAACCGTACGAACGTTATTCGGAATCACTGGGCTTAAAGCGTGCGTA6810.6255109257745405No Hit
TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA6560.6025479696154165No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA6230.5722368674853726No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA6160.5658072397608178No Hit
TACGTAGGACCCGAGCGTTGTCCGGAATTACTGGGTATAAAGGGTGCGCA5630.5171257727034748No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA5630.5171257727034748No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5420.49783688952981053No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA4970.4565035684433871No Hit
TACGGGCGGGGCGAGCGTTGTTCGGATTCATTGGGTGTAAAGGGTGCGAA4860.4463998677333725No Hit
TACAGAGGTGCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA4680.4298665392988032No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCTCA4620.42435542982061336No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA4590.42159987508151847No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA4570.4197628385887886No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA4350.3995554371687593No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA4130.37934803574873016No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA4080.37475544451690534No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA4080.37475544451690534No Hit
TACGTAGGCAGCAAACGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA3960.36373322556052573No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA3940.36189618906779586No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA3760.34536286063322647No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA3720.3416887876477666No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGGGCGTGTA3510.3223999044741024No Hit
AACGTAGGTGACAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA3390.3113776855177228No Hit
GACGTAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTA3280.30127398480770823No Hit
TACGAACTGTGCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA3240.2975999118222483No Hit
CACGGGGGTTGCAAGCGTTGTCCGGAATTACTAGGCGTAAAGTGCAGGTA3200.29392583883678847No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2960.27188140092402935No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA2960.27188140092402935No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGCGCGTA2940.2700443644312994No Hit
TACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2930.26912584618493446No Hit
CACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA2780.25534807248946No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCCCGCA2710.24891844476490527No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA2630.24157029879398553No Hit
CACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGCA2600.23881474405489067No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA2590.23789622580852568No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA2540.23330363457670084No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGCA2520.23146659808397094No Hit
TACGAAGGACCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2510.23054807983760597No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA2440.22411845211305123No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2430.22319993386668624No Hit
TACGTAGGGGGCGAGCGTTGTCCGAAGTTACTGGGCGTAAAGCGCGCGTA2420.2222814156203213No Hit
TACGTAAGGAGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCTCGTA2420.2222814156203213No Hit
CACGGGGGTTGCGAGCGTTGTCCGGAATTACTAGGCGTAAAGGGCAGGCA2370.2176888243884965No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA2310.21217771491030668No Hit
TACGGAGGTTGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGAGCGCA2200.20207401420029208No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA2070.19013327699754753No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGCA2060.1892147587511826No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1970.18094809453389793No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1890.1735999485629782No Hit
GACGTAGGGGGCGAGCGTTGTCCGAAGTGACTGGGCGTAAAGCGCACGTA1860.1708443938238833No Hit
TACGTACCGTCCAAACGTTATTCGGAATTACTGGGCTTAAAGGGTACGTA1820.16717032083842345No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCACGCA1810.1662518025920585No Hit
TACATAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTACGTA1790.16441476609932856No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAACGTA1770.16257772960659864No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA1740.15982217486750375No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1720.15798513837477382No Hit
TACGTAGGGGCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGCGCGCGCA1700.1561481018820439No Hit
AACGTAGGAGACAAGCGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGTA1700.1561481018820439No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA1630.14971847415748915No Hit
CACGGGGGTTGCGAGCGTTGTCCGGAATTACTAGGCGTAAAGGGCAGGTA1620.14879995591112416No Hit
TACAGAGGGTGCAAACGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA1520.13961477344747453No Hit
TACAGAGAGTGCAAACGTTGTTCGGAATCACTGGGCATAAAGCGCGCGTA1500.1377777369547446No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA1490.13685921870837964No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1470.1350221822156497No Hit
TACGGAGGGTGCGAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGTGTA1460.13410366396928475No Hit
TACGTAGGTGGCGAACGTTGTCCGGATTCACTGGGCGTAAAGCGCGCGCA1430.13134810923018986No Hit
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA1420.1304295909838249No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGTGCGTA1390.12767403624473No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA1380.12675551799836504No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1370.12583699975200008No Hit
TACAGAGGCGGCAAGCGTTGTTCGGAATTATTGGGCGTAAGGAGCACGTA1370.12583699975200008No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1350.12399996325927014No Hit
TACGTAGGTGACAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA1330.12216292676654021No Hit
TACATAGGTCCCAAGCGTTATCCGGATTTACTGGGCGTAAAGAGTCTGTA1330.12216292676654021No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA1290.11848885378108036No Hit
TACGGAGGTTCCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGAGCGCA1250.11481478079562049No Hit
TACAGAGGTGGCGAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA1240.11389626254925554No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGCA1240.11389626254925554No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGAGCTCGTA1220.11205922605652562No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1200.11022218956379569No Hit
TACGTAGGTGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCTTGTA1190.10930367131743073No Hit
GACGTAGGGGGCAAGCGTTACCCGGAATTACTGGGCGTAAAGGGAGCGTA1180.10838515307106575No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1180.10838515307106575No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA1160.10654811657833582No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA1150.10562959833197087No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTCACTGGGTGTAAAGGGCGCGCA1130.10379256183924095No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGCGCACGTA1110.101955525346511No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGCA1100.10103700710014604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)