FastQCFastQC Report
Tue 9 Sep 2025
Aerobic-seeding-sludge_merged.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAerobic-seeding-sludge_merged.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71765
Total Bases18.1 Mbp
Sequences flagged as poor quality0
Sequence length244-265
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA746810.406186859889917No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA36175.040061311224134No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA24083.355396084442277No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA23103.2188392670521844No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA21793.036299031561346No Hit
TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA15682.1849090782414824No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA15192.1166306695464363No Hit
TACGGAGGGTGCGAGCGTTATCCGGAATTACTGGGTTTAAAGGGTGCGTA15052.097122552776423No Hit
TACGTAGGTGACAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA14362.0009754058385005No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA13591.8936807636034276No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGCGTAAAGGGCGTCTA12351.7208945864975962No Hit
CACGTAGGTCGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCTTGTA10991.531387166446039No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA9691.350240367867345No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA9361.3042569497665994No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA8931.2443391625444158No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA8301.1565526370793562No Hit
TACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA6130.854176827144151No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA5940.8277015258134188No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA5550.7733574862398105No Hit
GACAGAGGAGCCAAGCGTTGTCCGGATTGACTGGGCGTAAAGCGCACGCA5530.7705706124155229No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA5380.7496690587333659No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA5360.7468821849090782No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5050.7036856406326204No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA4750.6618825332683063No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA4470.6228662997282798No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA4450.6200794259039922No Hit
CACGTAGGACGCAAGCGTTATCCGGAATTACTGGGCGTAAAGAGCGTGTA4440.6186859889918483No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA4340.6047516198704104No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA4260.59360412457326No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA4210.586636940012541No Hit
AACGTAGGGGGCGAGCGTTATCCGGAATCACTGGGCGTAAAGGGCGTCTA4150.5782763185396781No Hit
CACGTACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA3760.5239322789660699No Hit
CACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA3710.5169650944053508No Hit
CACGTAGGCACCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA3700.515571657493207No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA3530.49188322998676237No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3530.49188322998676237No Hit
GACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA3460.4821291716017557No Hit
CACGAACCGTACGAACGTTATTCGGAATTACTGGGCTTAAAGGGTTTGTA3450.48073573468961195No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA3440.4793422977774681No Hit
GACGAACCGTCCAAACGTTATTCGGAATCACTGGGCTTAAAGGGCGCGTA3370.4695882393924615No Hit
TACGGAAGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA3330.4640144917438863No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA3110.4333588796767226No Hit
TACGAAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA3100.4319654427645789No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA3080.42917856894029127No Hit
GACGTAGGAAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA3070.4277851320281474No Hit
AACGTAGGAGACAAACGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGTA3040.42360482129171606No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA3010.4194245105552846No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCATGTGCA2990.416637636730997No Hit
GACGAACCGAGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA2910.4054901414338466No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA2840.39573608304883995No Hit
GACGAGCTCTCCAAACGTTATTCGGTATCACTGGGCTTAAAGAGTTCGTA2840.39573608304883995No Hit
GACGAACCGTCCAAACGTTATTCGGTATCACTGGGCTTAAAGAGTTCGTA2820.39294920922455234No Hit
TACGAACTGTGCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA2820.39294920922455234No Hit
GACGAACCGTCCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA2780.38737546157597713No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2780.38737546157597713No Hit
TACGTAGGTGTCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCCCGCA2600.36229359715738874No Hit
CACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA2590.3609001602452449No Hit
GACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTA2570.3581132864209573No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA2470.34417891729951927No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2180.30376924684734896No Hit
TACGGAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA2160.3009823730230614No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA2150.2995889361109176No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA2140.2981954991987738No Hit
TACAGAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTACGCA2070.28844144081376716No Hit
TACGTAGGTGACGAGCGTTATCCGGAATTACTGGGCGTAAAGCGTACGCA2040.2842611300773357No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA2040.2842611300773357No Hit
GACGAACCGTCCGAACGTTATTCGGTATCACTGGGCTTAAAGAGTTCGTA2010.28008081934090434No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1900.26475301330732254No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA1850.2577858287466035No Hit
AACGTAGGAGACAAACGTTATCCGGAGTTACTGGGCGTAAAGGGCGTGCA1810.2522120810980283No Hit
TACAGAGGTGGCGAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA1790.2494252072737407No Hit
TACGAGAGGCCCAAACGTTATTCGGATTTACTGGGCTTAAAGAGTTCGTA1780.2480317703615969No Hit
TACGAACTGTGCGAACGTTACTCGGAATCACTGGGCTTACAGGGTGCGTA1700.23688427506444645No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1670.23270396432801504No Hit
TACGTAGGTGACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCACGCA1660.23131052741587124No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1620.22573677976729603No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGTA1590.22155646903086462No Hit
TACGGAGGGTGCGAACGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA1590.22155646903086462No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGGGCGTGTA1590.22155646903086462No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1560.21737615829443324No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1540.21458928447014564No Hit
TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA1520.21180241064585803No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1490.2076220999094266No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA1400.19508116770013237No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA1390.19368773078798857No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1360.18950742005155716No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1350.18811398313941335No Hit
TACAGAGGGGGCTAGCGTTGTTCGGAATGACTGGGCGTAAAGGGCGCGTA1340.18672054622726955No Hit
GACGAACCACACAAACGTTATTCGGAATTACTGGGCTTAAAGGGTGCGTA1320.18393367240298197No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1290.17975336166655054No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1270.17696648784226293No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA1260.17557305093011913No Hit
TACGTAGGGTCCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGTTGCGT1260.17557305093011913No Hit
TACGAAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1210.16860586636940011No Hit
TACGAAGGGGACTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA1200.16721242945725634No Hit
TACGTACCGTCCAAACGTTATTCGGAATTACTGGGCTTAAAGGGTACGTA1160.16163868180868113No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGCA1140.1588518079843935No Hit
TACGAAGGTCCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1090.15188462342367448No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA1070.1490977495993869No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA1070.1490977495993869No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA1040.1449174388629555No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA1010.14073712812652406No Hit
CACGTAGGAGGCAAACGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA960.13376994356580504No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA950.13237650665366127No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA940.13098306974151747No Hit
TACGTAGGGTCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGCGCGCGCA930.12958963282937366No Hit
GACGAACCGTACGAACGTTATTCGGAATTACTGGGCTTAAAGGGTGCGTA900.12540932209294225No Hit
TACGGAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA860.11983557444436703No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA860.11983557444436703No Hit
TACGGAGGGTGCGAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA850.11844213753222323No Hit
GACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGTA840.11704870062007942No Hit
TACGAAGGGTGCAAGCGTTATTCGGAATGACTGGGCGTAAAGGGTGTGTA830.11565526370793562No Hit
GACGAACCGTGCAAACGTTATTCGGAATCACTGGGCATAAAGGGCGCGTA830.11565526370793562No Hit
AACGTAGGAGGCGAGCGTTATCCGGAGTTACTGGGCGTAAAGCGCGTGTA800.11147495297150421No Hit
TACGTAGGGTGCAAGCATTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA780.10868807914721662No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCATCTA770.10729464223507282No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA750.10450776841078521No Hit
AACGTAGGAGGCAAGCGTTATCCGGAGTTACTGGGCGTAAAGCGTGCGTA740.1031143314986414No Hit
TACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCATAAAGCGCGCGTA730.10172089458649759No Hit
TACGTAAGGGGCAAGCGTTGTCCGGAATCACTGGGCGTAAAGCGCACGTA720.10032745767435379No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA720.10032745767435379No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCATTGGGCTTAAAGGGCGCGTA720.10032745767435379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)