FastQCFastQC Report
Tue 9 Sep 2025
Aerobic-seeding-sludge_S114_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAerobic-seeding-sludge_S114_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73105
Total Bases18.3 Mbp
Sequences flagged as poor quality0
Sequence length249-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1300.17782641406196567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)